Cloning and Sequencing of Desulfurization Operon froma Newly Isolated Bacterium Rhodococcus FMF
نویسندگان: ثبت نشده
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منابع مشابه
Study of Desulfurization Rate in Rhodococcus FMF Native Bacterium
The Rhodococcus FMF (R. FMF) native bacterium was isolated from soil contaminated with oil in Tabrizrefinery. This bacterium carries three genes sox (dszA, B, C) on its genomic DNA. Preliminary studieshave proved that R. FMF strain possess desulfurization activity. In this work soxA and B genes wereamplified by PCR, after designing a pair of suitable primers. Analysis of PCR p...
متن کاملIsolation and Identification of a Sulfide/Sulfoxide Monooxygenase Gene from a Newly Isolated Rhodococcus Sp. Strain FMF
Rhodococcus FMF is a gram-positive bacterium isolated for the first time from soil samples of Tabriz refinery in Iran. This microorganism is able to catabolize dibenzothiophene to 2-hydroxybiphenyl and inorganic sulfur without the destruction of carbon-carbon bonds. Three structural genes, dszA, dszB, and dszC have been characterized and shown to be responsible for this phenotype. In this work,...
متن کاملSulfur-selective desulfurization of dibenzothiophene and diesel oil by newly isolated Rhodococcus sp. strains.
New desulfurizing bacteria able to convert dibenzothiophene into 2-hydroxybiphenyl and sulfate were isolated from contaminated soils collected in Mexican refineries. Random amplified polymorphic DNA analysis showed they were different from previously reported Rhodococcus erythropolis desulfurizing strains. According to 16S rRNA gene sequencing and fatty acid analyses, these new isolates belonge...
متن کاملisolation and identification of a sulfide/sulfoxide monooxygenase gene from a newly isolated rhodococcus sp. strain fmf
rhodococcus fmf is a gram-positive bacterium isolated for the first time from soil samples of tabriz refinery in iran. this microorganism is able to catabolize dibenzothiophene to 2-hydroxybiphenyl and inorganic sulfur without the destruction of carbon-carbon bonds. three structural genes, dsza, dszb, and dszc have been characterized and shown to be responsible for this phenotype. in this work,...
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The current study was aimed at isolating and identifying the halophilic and halotolerant bacteria which can produce mercuric reductase in Gavkhuni wetland in Iran. Moreover, tracking and sequencing merA gene and kinetic properties of mercuric reductase in the selected strain were performed in this study. Soil samples were taken from Gavkhuni wetland and cultured in nutrient agar medium...
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Bacillus subtilis RB14 is a producer of the antifungal lipopeptide iturin A. Using a transposon, we identified and cloned the iturin A synthetase operon of RB14, and the sequence of this operon was also determined. The iturin A operon spans a region that is more than 38 kb long and is composed of four open reading frames, ituD, ituA, ituB, and ituC. The ituD gene encodes a putative malonyl coen...
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عنوان ژورنال
دوره 2 شماره 1
صفحات 19- 24
تاریخ انتشار 2004-01-01
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